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DNA Barcoding of Israeli Indigenous and Introduced Cichlids

The Israeli Journal of Aquaculture – Bamidgeh 61(2), 2009, 83-88.

Full article available to e-journal subscribers only at http://www.siamb.org.il
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DNA Barcoding of Israeli Indigenous and Introduced Cichlids

Andrey Shirak, Miri Cohen-Zinder, Renata M. Barroso, Eyal Seroussi, Micha Ron and Gideon Hulata*

Institute of Animal Science, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan 50250, Israel

(Received 7.12.06, Accepted 1.1.09)

Key words: barcoding, taxonomic analysis, cichlids, cytochrome oxidase subunit I, variability, commercial species, conventional taxonomy

Abstract
The objectives of this study were barcoding and taxonomic analysis of the five tilapiine species (Oreochromisaureus, O.niloticus, O.mossambicus, Sarotherodon galilaeus, and Tilapia zillii), two tilapia hybrid strains (Florida red tilapia and Philippine red tilapia), and two endemic wild cichlids (Tristramella simonis and Astatotilapia flaviijosephi) available in Israel, as well as O. urolepis hornorum. Cytochrome oxidase subunit I (COI) 619 bp sequence traces of 104 individuals were assembled, aligned, and compared (GenBank project GI 209553463). The DNA sequences of two hybrid strains were identical to those of O. hornorum and O. aureus. Absence of intra-specific variability was detected in the commercially used species, O. aureus, S. galilaeus, O. mossambicus, and O. urolepis hornorum. Two DNA sequence variants were detected in O. niloticus originating from Ghana and Egypt. In contrast, 2-3 variants were detected in the DNA of each of the non-commercial species. Amino-acid sequences were identical in all “true tilapias” and different from the sequences in the endemic cichlids. As a whole, the protein phylogenetic tree fitted the expected conventional taxonomy as opposed to the respective DNA-based tree. Sequences FJ348047-FJ348150 were submitted to GenBank via the BOLD database (identical to FISH001-08 - FISH104-08 in this database).

* Corresponding author. E-mail: vlaqua@volcani.agri.gov.il

Tags: analysis, barcoding, cichlids, commercial, conventional, cytochrome oxidase subunit I, species, taxonomic, taxonomy, variability

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Shirak_et_al_2009_DNA_barcoding_Israeli_cichlids-1.pdf, 125 KB

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Permalink Reply by Dan Mires on February 13, 2010 at 8:55pm: Looks good. Except that the name of the IJA, Vol. and pages are missing. All these are necessary to cite our articles.; ▶ Reply to This

Permalink Reply by Darren Okimoto on February 14, 2010 at 8:05am: Aloha Benny:
I agree with Dan. You'll need to include the journal citation information or the readers will not know how to track this down.; ▶ Reply to This

Permalink Reply by Benny Ron on February 14, 2010 at 8:19am: Check it out now and let me know if OK; ▶ Reply to This

Permalink Reply by Gideon Hulata on February 14, 2010 at 12:06pm: Thanks. Now it looks OK.
Gidi

Benny Ron said:
Check it out now and let me know if OK; ▶ Reply to This

Permalink Reply by Benny Ron on February 15, 2010 at 9:32pm: I hope that Dan Mires Like this way of publishing the articles.; ▶ Reply to This

Permalink Reply by Benny Ron on February 15, 2010 at 9:45pm: The question now is if one "subscribe" or "register" to the Open Access journal since subscription is a business model where a customer must pay a subscription price to have access to the product/service?; ▶ Reply to This